Journal: Scientific Reports

Article Title: Nuclear receptor 4A1 ameliorates UUO-induced renal fibrosis by inhibiting the PI3K/AKT pathway

doi: 10.1038/s41598-024-76219-x

Figure Lengend Snippet: NR4A1 may regulate the PI3K/Akt signaling pathway to improve renal fibrosis in UUO mice. ( A ) Western blot to examine PI3K/Akt expression in mice (n = 3). ( B ) Western blot to examine PI3K/Akt expression in NR4A1 knocking down mice (n = 3). ( C ) Western blot to examine PI3K/Akt expression in HK-2 (n = 3). ( D ) Western blot to examine PI3K/Akt expression in HK-2 (n = 3). The data are presented as the mean ± SD, * P < 0.05 vs. the sham/sh-con/Con group. # P < 0.05 vs. the sh-NR4A1/TGF-β1 group.

Article Snippet: Shake the bed at room temperature for 1 h and incubate, the primary antibody α-SMA (1:1000), Vimentin (Abcam,1:500,Cat#: ab92547), Collagen I (Proteintech,1:300,Cat #:14695-1-AP), VEGFA (1:500), CD105 (1:500), TNF-α (1:100), Bcl-2 (Proteintech,1:500,Cat #:26593-1-AP), Bax (Proteintech,1:500,Cat #:50599-2-Ig), NF-κB(Proteintech,1:500,Cat#:10745-1-AP), GAPDH (Proteintech, 1:3000, Cat#:10494-1-AP), PI3K(Proteintech, 1:500, Cat#:60255-1-1g), PI3K (phospho Tyr467/199, Immunoway, 1:500, Cat#:YP0225), Akt (Proteintech, 1:500, Cat#:60203-2-Ig), phospho-Akt (Ser473, Proteintech, 1:500, Cat#:60203-2-Ig) at 4 °C overnight.

Techniques: Western Blot, Expressing